Fig. 3

Circ0120816 acted as a sponge for miR-1305. a The binding sequence between circ0120816 and miR-1305 was predicted by TargetScan 7.0. b Luciferase reporter assay was used to confirm the relationship between circ0120816 and miR-1305. **P < 0.001, compared with miR-NC. miR-NC: miR-1305 negative control, circ-WT: circ0120816 wild type, circ-Mut: circ0120816 mutant type. c RIP analysis showed that circ0120816 was abundantly pulled down by antiAgo2 antibodies when transfected with miR-1305 mimics in ESCC cells compared with the miR-1305 NC and IgG group. **P < 0.001, compared with miR-NC. miR-NC: miR-1305 negative control, IgG: negative control, Ago2: anti-Argonaute2 antibody. d RT-qPCR analysis showed that the expression of miR-1305 was decreased in ESCC tissues. Normal: adjacent healthy tissues, Tumor: ESCC tissues. e Pearson’s correlation analysis showed that miR-1305 was negatively associated with circ0120816. Each cellular experiment was independently repeated three times, and the data collected were illustrated in the format of mean ± standard deviation (SD)