Fig. 4

Circ0120816 facilitated ESCC progression by sponging miR-1305. a RT-qPCR analysis of the expression of circ0120816 and miR-1305 in ESCC cell lines after transfecting with circ0120816 overexpression plasmid, circ0120816 siRNA or miR-1305 inhibitor. b CCK-8 assay was used to determine the viability of ESCC cell lines after transfection. c BrdU assay was conducted to evaluate the proliferation of ESCC cell lines after transfection. d Cell adhesion assay was employed to determine the cell adhesion ability of ESCC cell lines after transfection. e Cell cycle assay was applied to evaluate the cell cycle in ESCC cell lines after transfecting with a flow cytometry. f The expression of cyclinB1, ICAM1 and VCAM1 was detected in ESCC cell lines after transfection by western blot assay. g Caspase 3 activity assay indicates the cell apoptosis of ESCC cell lines after transfection. h The expression of Cleaved PARP, Bax and Cleaved Caspase-3 was detected in ESCC cell lines after transfecting with western blot assay. *P < 0.05, **P < 0.001, compared with the blank control group. ^#P < 0.05, ^##P < 0.001, compared with the co-transfection group of si-circ0120816 plus miR-1305 inhibitor. CON: blank control, NC: negative control, OE-circ: circ0120816 overexpression plasmid, si-circ: circ0120816 siRNA, inhibitor: miR-1305 inhibitor, si-circ + inhibitor: circ0120816 siRNA plus miR-1305 inhibitor. Each experiment was independently repeated three times, and the data collected were displayed in the format of mean ± standard deviation (SD)