Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: Overexpression of protein regulator of cytokinesis 1 facilitates tumor growth and indicates unfavorable prognosis of patients with colon cancer

Fig. 5

Silencing of PRC1 arrested more cells in G2/M phase and induces cell apoptosis in HCT116 and SW480 cells. a Cell cycle distribution of HCT116 and SW480 cells in the three groups (shControl, shPRC1-1, and shPRC1-2). b Percentage of cells in cell cycle phase G0/GA, S, and G2/M. c The expression levels of cell cycle related proteins (Cdc25c, Cdc2, Cyclin B1, P21, and P27) were detected by western blotting. d Cell apoptosis in HCT116 and SW480 cells detected by flow cytometry. e Apoptosis rate in HCT116 and SW480 cells in the three groups. f The expression level apoptosis related proteins (pro caspase 3, cleaved caspase 3, full length PARP, Bcl2, Bcl-xl, and Bax) was detected by western blotting. Data were expressed as the mean ± standard deviation (mean ± SD). *P < 0.05, **P < 0.01, # P < 0.001

Back to article page