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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis

Fig. 2

Effect of TGF-β2 and NEAT1 knockdown on the progression of SRA01/04 cells. SRA01/04 cells were transfected with si-NC or si-NEAT1, and then treated with 10 ng/mL TGF-β2. a QRT-PCR analysis showed that si-NEAT1 could inhibit NEAT1 expression (P < 0.0001) in TGF-β2-treated SRA01/04 cells. b MTT assay indicated that NEAT1 silencing reduced the proliferation of TGF-β2-treated SRA01/04 cells (P < 0.0001). c, d Transwell assay revealed that NEAT1 knockdown suppressed the migration and invasion of TGF-β2-treated SRA01/04 cells (100 μM) (P < 0.001, P < 0.0001). e WB analysis showed that silenced NEAT1 enhanced E-cadherin protein level, while inhibited Vimentin and α-SMA protein levels (P < 0.001, P < 0.0001). **P < 0.01, ***P < 0.001, ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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