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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis

Fig. 3

NEAT1 interacted with miR-486-5p. a The sequences of NEAT1-WT and NEAT1-MUT were shown. b Dual-luciferase reporter assay suggested that the luciferase activity of NEAT1-WT could be inhibited by miR-486-5p (P < 0.0001). c RIP assay showed that the enrichment of NEAT1 and miR-486-5p was enhanced in Ago2 (P < 0.0001). d Biotin-labeled RNA pull-down assay suggested that NEAT1 enrichment was increased in the bio-miR-486-5p probe (P < 0.001). e QRT-PCR analysis indicated that NEAT1 expression was decreased by si-NEAT1 (P < 0.05) and promoted by NEAT1 overexpression vector (P < 0.0001) in TGF-β2-stimulated SRA01/04 cells. f QRT-PCR analysis showed that miR-486-5p expression was increased by NEAT1 knockdown (P < 0.0001) and reduced by NEAT1 overexpression (P < 0.0001) in TGF-β2-stimulated SRA01/04 cells. *P < 0.05, ***P < 0.001, ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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