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Fig. 4 | Cancer Cell International

Fig. 4

From: LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis

Fig. 4

Effects of NEAT1 silencing and miR-486-5p inhibitor on the progression of TGF-β2-stimulated SRA01/04 cells. SRA01/04 cells were co-transfected with si-NEAT1 and anti-miR-486-5p or anti-miR-NC, and then treated with 10 ng/mL TGF-β2. a QRT-PCR analysis suggested that miR-486-5p inhibitor reversed the promotion effect of NEAT1 silencing on miR-486-5p expression (P < 0.0001). MTT assay (b), and transwell assay (c, d) showed that miR-486-5p inhibitor reversed the inhibition effect of NEAT1 knockdown on the proliferation (P < 0.0001), migration (P < 0.001) and invasion (P < 0.0001) (100 μM) of TGF-β2-stimulated SRA01/04 cells. e WB analysis indicated that the regulation of NEAT1 silencing on the protein levels of E-cadherin, Vimentin and α-SMA could be reversed by miR-486-5p inhibitor (P < 0.0001) in TGF-β2-stimulated SRA01/04 cells. ***P < 0.001, ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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