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Fig. 3 | Cancer Cell International

Fig. 3

From: EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer

Fig. 3

Silencing of EZH2 inhibits proliferation, migration, and invasion, and induces apoptosis in OC cells. a The mRNA expression pattern of EZH2 after treatment with shRNA-EZH2*1, shRNA-EZH2*2 and shRNA-EZH2*3 evaluated by RT-qPCR; b The protein expression pattern of EZH2 after treatment with shRNA-EZH2*1, shRNA-EZH2*2 and shRNA-EZH2*3 assessed by western blot analysis. In panels C-G, SKOV3 cells were treated with shRNA-NC and shRNA-EZH2 plasmids. c The proliferation ability of each group tested by EdU staining (×200). d The cell migration ability determined by Transwell assay (×100). e The invasive ability of cells in each group examined by Transwell assay (×100). f The cell apoptosis in each group measured by flow cytometry. g The protein expression pattern of CyclinD1, CDK2, MMP-2, MMP-9, Cleaved Caspase-3, Bax and Bcl-2 in cells assessed by western blot analysis. *p < 0.05 vs. cells transfected with shRNA-NC. The measurement data were expressed as mean ± standard deviation. The paired-designed data was compared using the paired t-test. The one-way analysis of variance was adopted for comparison among multiple groups, followed by the Tukey’s post hoc test. The experiment was conducted 3 times independently

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