Fig. 4

EZH2 down-regulates miR-139 expression through promoting the methylation of miR-139 promoter by recruiting H3K27me3. a miR-139 expression pattern in the OC tissues and adjacent normal tissues evaluated by RT-qPCR. b The correlation between miR-139 expression pattern and EZH2 expression pattern as assessed by Pearson correlation analysis. In panels C and D, SKOV3 cells were treated with shRNA-EZH2, oe-EZH2 alone or in the presence of DZNep. c The miR-139 expression pattern in cells determined by RT-qPCR. d The enrichment of H3K27me3 in the miR-139 promoter region of cells measured by ChIP. *p < 0.05 vs. adjacent normal tissues or the shRNA-NC group, ^#p < 0.05 vs. the oe-NC group, ^@p < 0.05 vs. the oe-EZH2 + DMSO group. The measurement data were expressed as mean ± standard deviation. The paired-designed data was compared using paired t-test. The one-way analysis of variance was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was conducted 3 times independently