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Fig. 7 | Cancer Cell International

Fig. 7

From: EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer

Fig. 7

The induction of EZH2, LPA1 and the TGF-β signaling pathway are responsible for accelerated OC cell proliferation, migration, invasion and delayed apoptosis. a The mRNA expression of LPA1 after treatment with shRNA-LPA1*1, shRNA-LPA1*2 and shRNA-LPA1*3 evaluated by RT-qPCR; b The protein expression pattern of LPA1 after treatment with shRNA-LPA1*1, shRNA-LPA1*2 and shRNA-LPA1*3 assessed by western blot analysis. c Cell proliferation ability detected by EdU assay (×200). d Cell migration ability determined by Transwell assay (×100). d Cell invasive ability examined by Transwell assay (×100). f Cell apoptosis monitored by flow cytometry. g CyclinD1, CDK2, MMP-2, MMP-9, Cleaved Caspase-3, Bax and Bcl-2 related protein expression pattern examined by western blot analysis. h The protein expression pattern of TGF-β signaling pathway-related factors measured by western blot analysis. *p < 0.05 vs. sh-NC group, #p < 0.05 vs. sh-LPA1 + DMSO group, ^p < 0.05 vs. sh-EZH2 + inhibitor NC group, %p < 0.05 vs. sh-EZH2 + oe-L-NC group. The measurement data were expressed as mean ± standard deviation. The statistical significance of the data between two groups was calculated using the unpaired t-test. The one-way analysis of variance was adopted for comparison among multiple groups, followed by the Tukey’s post hoc test. The experiment was conducted 3 times independently

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