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Fig. 4 | Cancer Cell International

Fig. 4

From: LncRNA LEF1-AS1 promotes metastasis of prostatic carcinoma via the Wnt/β-catenin pathway

Fig. 4

LEF1-AS1 promotes Wnt/β-catenin pathway activation via FZD2. a Compared to that observed in the normal cell line RWPE, Wnt/β-catenin signalling pathway activation was significantly greater in PC3 and DU145 cells. ***P < 0.001. b p-GSK3β (ser9), β-catenin, MMP-7 and c-myc levels were increased in PC3 cells transfected the with LEF1-AS1 overexpression plasmid and decreased in cells transfected with the siLEF1-AS1. (C) FZD2 expression was positively correlated with that of LEF1-AS1 in AIPC, as determined using the starBase 3.0 database. d FZD2 expression was measured in AIPC cells treated with siLEF1-AS1 or the LEF1-AS1 plasmid by RT-qPCR and western blot analysis. *P < 0.05. e Schematic graph of the LEF1-AS1 binding site deletion in the FZD2 promoter (predicted using LongTarget). ***P < 0.001. f RegRNA in silico prediction of the LEF1-AS1 secondary structure interacting with the c-myb transcription factor (protein), with a calculated binding free energy of − 41.1 kcal/mol. *P < 0.05. g Multiple binding sites for the transcription factor c-myb are predicted in the FZD2 promoter region (JASPAR database). The ChIP assay results confirmed the occupancy of C-myb in the FZD2 promoter. *P < 0.05. h FZD2 expression was measured by RT-qPCR and western blot assays. *P < 0.05

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