Fig.6
From: CD4 T-cell immune stimulation of HER2 + breast cancer cells alters response to trastuzumab in vitro
ELISA analysis of TNF-α expression in trastuzumab treated HER2 + breast cancer. a Fold change of TNF-α ELISA analysis from day 0 to 7 reveals an increase in TNF-α expression in trastuzumab treated cells cultured with CD4 + T-cells (p = 0.02). b Western blot of TNF-α and β-actin expression in BT474, SKBR3, MDA-MB-453 and MDA-MB-231 cell lines. c High TNF-α expression was observed in the BT474 cell line. Moderate TNF-α expression was observed in the SKBR3 cell line. Low TNF-α expression was observed in MDA-MB-453 and MDA-MB-231 cell lines. β-actin was also assayed and used to normalize protein expression