Skip to main content
Fig. 1 | Cancer Cell International

Fig. 1

From: TRIM3 inhibits P53 signaling in breast cancer cells

Fig. 1

TRIM3 negatively correlates P53 signaling in breast cancer cells. a The KMPLOT online analysis showed that TRIM3 related to good prognosis in all breast cancer patients. b The KMPLOT online analysis showed that TRIM3 tend to relate to good prognosis in P53 wild type breast cancer patients. c TRIM3 knocking down efficiency in MCF-7 cells. MCF-7 cells were transfected with TRIM3 siRNA. After 48 h, TRIM3 protein was determined by Western blot. Actin was used as internal control. d TRIM3 depletion increased P53 protein levels in MCF-7 cells. MCF-7 cells were transfected with siControl or siTRIM3. After 48 h, cells were harvested for western blot analysis. TRIM3 and P53 protein levels were determined by Western blot. Actin was used as internal control. e TRIM3 depletion increased P53 target gene expression in MCF-7 cells. MCF-7 cells were transfected with siControl or siTRIM3. After 48 h, total RNA was extracted for gene expression analysis. Each group was done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for target gene expression comparison

Back to article page