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Fig. 3 | Cancer Cell International

Fig. 3

From: TRIM3 inhibits P53 signaling in breast cancer cells

Fig. 3

TRIM3 depletion activates P53 signaling, inhibits cell growth in MDAMB175 breast cancer cells. a TRIM3 depletion increased P53 protein levels in both vehicle and cisplatin-treated conditions. MDAMB175 cells were transfected with siControl or siTRIM3. 1 ug/ml Cisplaitn was added to treat the cells for 6 h. Then cells were harvested for western blot analysis. TRIM3 and P53 protein levels were determined by Western blot. Actin was used as internal control. b TRIM3 depletion increased P53 target gene expression in both vehicle and cisplatin-treated conditions. MDAMB175 cells were transfected with siControl or siTRIM3. After 48 h, 1 ug/ml Cisplaitn was added to treat the cells for 6 h. total RNA was extracted for gene expression analysis. Each group was done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for target gene expression comparison. c TRIM3 depletion inhibited MDAMB175 cells proliferation. MDAMB175 cells were transfected with siControl or siTRIM3. After 24 h, the WST assay was used to determine the cellar metabolic activity at indicated time points after infection. Experiments were done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for cell growth comparison. d, e TRIM3 depletion inhibited the number of EdU positive breast cancer cells. MDAMB175 cells were transfected with siControl or siTRIM3. After 24 h, EdU was added into the medium for 2 h incubation. The absolute cell number was counted to indicate cell proliferation activity

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