Fig. 2

FOXM1 expression is closely associated with docetaxel-induced inhibition of migration and invasiveness in PCa cells. a, b VCaP and DU145 cells were transfected with FOXM1 plasmid or NC, using lipofectamine 2000 in 96-well plates. At 48 h post-transfection, cells were treated with 12.5 nM docetaxel (DOC) for 72 h. Wound closure assays and transwell invasion assays were undertaken to analyze migration and invasiveness of parental cells. c, d VCaP-DR and DU145-DR cells were treated with siFOXM1 and DOC (25 nM). Transwell invasion assays assessed the invasive capabilities of the cells. e Transwell invasion assays detected the invasive abilities of FOXM1 stable-knockout DU145-DR cells or in combination with DOC (25 nM) for 24 h, 48 h, and 72 h. Data represent the mean ± SD of three independent experiments. ^*p < 0.05, ^**p < 0.01