Table 3 The specific primer sequences for selected lncRNAs and mRNAs
Gene name | Primer sequence (5′ to > 3′) | Annealing (°C) | Length product (bp) |
|---|---|---|---|
HAGLR (lnc-AC009336.1-2) | CAGACTCAGCAGATACTC TCATCTCCTCTTCCTACC | 62 | 204 |
CDKN2B-AS1 (lnc-MTAP-1) | TATTCCTGGCTCCCCTCGTC TGACCTCGCTTTCCTTTCTTCC | 61 | 185 |
PDCD4-AS1 (lnc-BBIP1-1) | GTCCTACCTCCCCCACTAAC TGGAACAATCCCTCCCACA | 58 | 181 |
LINC00675 (lnc-PIRT-1) | TTAGAACCAACCACAAGCACCA AGCCAGTGAGGAGAAATAGCAAC | 60 | 88 |
FAM120AOS (nc-FAM120AOS-4) | GCAGAACACCAAAGGAGACC ATTTTTGCATCAGCCCAAAG | 60 | 195 |
LOC101929340 (lnc-NRGN-1) | TCAACCAACAGGCATCAGAA CCGCAAATCCAGGTAAGAAC | 58 | 143 |
ABCC13 (lnc-RBM11-5) | AGGAATCAAGAGAGGCAAAAAGC GAGTGGGCTAGTGAAGGACAA | 59 | 162 |
LOC102724156 (lnc-CDK20-8) | GGACACCCTAGAGGCAGAGA TCAAACTTGTGTGCTGAAGAGA | 62 | 150 |
CYCS | GGCGGCTGTGTAAGAGTATCCA AACCTTACCCCAGTGGTGCTC | 60 | 192 |
CASP9 | AACCTTACCCCAGTGGTGCTC ATCTGCATTTCCCCTCAAACTCTCA | 62 | 138 |
BCL2 | AACATCGCCCTGTGGATGACT GGCAGGCATGTTGACTTCACTT | 62 | 223 |
CASP7 | GGACCGAGTGCCCACTTATC TCGCTTTGTCGAAGTTCTTGTT | 60 | 89 |
BAD | GGAGGATGAGTGACGAGTTTG TTCGGGATGTGGAGCGAAG | 60 | 184 |
GAPDH | TGAAGTCGCAGGAGACAACC TGCCGCCTGGAGAAACC | 60 | 121 |