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Fig. 2 | Cancer Cell International

Fig. 2

From: Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma

Fig. 2

SUV39H2 contributes to accelerated EMT and migration of OS cells. MG63 cells were transfected with sh-NC, sh1-SUV39H2 and sh2-SUV39H2. a Transfection efficiency of SUV39H2 evaluated using RT-qPCR. b The positive rates of N-cadherin, E-cadherin and Vimentin detected using Immunofluorescence. c The protein expression of SUV39H2, E-cadherin and Vimentin normalized to GAPDH in MG63 cells detected by Western blot analysis. d Cell migration and invasion tested by the means of Transwell assay. e Cell apoptosis detected by flow cytometry. *p < 0.05 vs. sh-NC-transfected MG63 cells. Measurement data are expressed as mean ± standard deviation derived from at least 3 independent experiments. One-way ANOVA was conducted for comparison among multiple groups, followed by Tukey’s post hoc test

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