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Fig. 3 | Cancer Cell International

Fig. 3

From: Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma

Fig. 3

SUV39H2 promotes the expression of LSD1, thereby accelerating EMT and migration of OS cells. MG63 cells were transfected with oe-NC, oe-SUV39H2, oe-SUV39H2 + sh-NC, oe-SUV39H2 + sh1-LSD1 or oe-SUV39H2 + sh2-LSD1. a Venn diagram with each circle respectively displaying the OS-related genes, genes that interacted with SUV39H2 through GeneCard and genes that were positively associated with SUV39H2 in OS by UALCAN. The middle part refers to the intersection. b Expression of LSD1 in OS samples of TCGA database. c The co-expression relationship between SUV39H2 and LSD1 in OS samples obtained using Chipbasev2.0 website. d LSD1 mRNA expression in OS tissues and paracancerous tissues detected using RT-qPCR. e LSD1 protein expression normalized to GAPDH in OS tissues and paracancerous tissues detected using Western blot analysis. *p < 0.05 vs. paracancerous tissues, n = 58. f The LSD1 mRNA expression in MG63 cells after overexpressing SUV39H2 detected using RT-qPCR. g H3K4m3 enriched in LSD1 promoter region through UCSC website. h H3K4m3 enrichment in LSD1 promoter region evaluated using ChIP assay. i The transfection efficiency of LSD1 in MG63 cells determined by RT-qPCR. j EMT-related protein expression in MG63 cells detected using immunofluorescence. k The protein expression of E-cadherin, N-cadherin, Vimentin, LSD1 and SUV39H2 normalized to GAPDH in each group detected using Western blot analysis. l The cell migration capacity evaluated using Transwell assay. m The cell apoptosis detected using flow cytometry. *p < 0.05 vs. oe-NC transfected MG63 cells and #p < 0.05 vs. MG63 cells co-transfected with oe-SUV39H2 + sh-NC. Measurement data were expressed as mean ± standard deviation derived from at least 3 independent experiments. Paired t-test was used for the data of OS tissues and paracancerous tissues, and unpaired t-test was used for the other two groups. The data comparison between multiple groups was performed using one-way ANOVA

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