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Fig. 4 | Cancer Cell International

Fig. 4

From: Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma

Fig. 4

LSD1 promotes EMT and migration of OS cells by inhibiting the expression of CDH1. a The interactive relationship of OS-related genes analyzed using STRING. The x-axis indicates the number of interactions shared by, and the y-axis indicates the gene name. b Heatmap displaying genes in OS samples of TCGA database predicted using UALCAN. The x-axis represents the sample and y-axis represents the name of the gene. c Expression of CDH1 in OS samples of TCGA database from UALCAN. d The co-expression relationship between LSD1 and CDH1 identified using Chipbasev2.0. e Expression of CDH1 in OS tissues and paracancerous tissues from patients with OS examined using RT-qPCR. f Expression of CDH1 normalized to GAPDH in OS tissues and paracancerous tissues from patients with OS examined using Western blot analysis. *p < 0.05 vs.. paracancerous tissues, n = 58. g CDH1 expression after overexpressing LSD1 measured using RT-qPCR. h High enrichment of H3K4m3 in CDH1 promoter region found through the UCSC website. i The enrichment of H3K4m3 in CDH1 promoter region assessed using ChIP assay. j EMT-related protein expression tested using immunofluorescence. k N-cadherin, E-cadherin and Vimentin protein expression normalized to GAPDH measured using Western blot analysis. l Cell migration capacity detected using Transwell assay. m Cell apoptosis detected by flow cytometry. *p < 0.05 vs. oe-NC transfected cells. #p < 0.05 vs. cells co-transfected with oe-LSD1 + sh-NC. Measurement data were expressed as mean ± standard deviation derived from at least 3 independent experiments. Paired t-test was used for the data of OS tissues and paracancerous tissues, and unpaired t-test was used for the other two groups. The data comparison between multiple groups was performed using one-way ANOVA

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