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Table 1 Main characteristics of the eligible studies

From: Identification of RASSF1A promoter hypermethylation as a biomarker for hepatocellular carcinoma

Study Country No. of patient Case Control Control sample Sample type Detection method
M T M T
Yu, 2002 [17] China 33 29 29 24 29 Non-tumor Tissue MSP
0 4 Normal
Zhang, 2002 [18] China 94 70 82 7 10 Non-tumor Tissue MSP
0 12 Normal
Lee, 2003 [19] Korea 144 40 60 2 86 Non-tumor Tissue MSP
Undraga, 2003 [20] USA 24 14 15 5 9 Non-tumor Tissue MSP
Zhong, 2003 [21] China 23 23 23 7 23 Non-tumor Tissue BSP
Lehmann, 2005 [22] Germany 131 40 41 53 83 Non-tumor Tissue qMSP
16 28 Normal
Park, 2005 [23] Korea 27 12 27 0 27 Non-tumor Tissue MSP
Yeo, 2005 [24] China 50 17 40 0 10 Normal Blood MSP
Calvisi, 2006 [25] USA 80 52 80 32 80 Non-tumor Tissue MSP
Gioia, 2006 [26] Italy 84 26 26 81 95 Non-tumor Tissue MSP
11 13 Normal
Oh, 2007 [27] Korea 32 23 25 9 24 Non-tumor Tissue MSP
2 7 Normal
Zhang, 2007 [28] China 100 35 50 3 50 Normal Blood MSP
Zhang, 2007 [29] China 56 44 50 24 50 Non-tumor Tissue MSP
0 6 Normal
Chan, 2008 [30] China 126 59 63 37 63 Non-tumor Blood MSRE-qPCR
Chang, 2008 [31] China 70 12 19 4 17 Non-tumor Tissue MSP
7 26 3 16 Blood
Nishida, 2008 [32] Japan 99 66 77 43 77 Non-tumor Tissue MSRE-qPCR
10 22 Normal
Su, 2008 [33] China 50 50 50 50 50 Non-tumor Tissue MSP
Lou, 2009 [34] China 86 57 60 54 81 Non-tumor Tissue MSP
0 5 Normal
Hu, 2010 [35] China 45 31 35 18 35 Non-tumor Tissue MSP
14 35 0 10 Normal Blood
Formeister, 2010 [36] USA 49 43 43 31 45 Non-tumor Tissue MSP
Feng, 2010 [37] USA 65 10 40 1 25 Normal Tissue Methylight
Saelee, 2010 [38] Thailand 29 25 29 3 29 Normal Tissue MSP
Hua, 2011 [39] China 55 30 47 9 47 Non-tumor Tissue MSRE-qPCR
30 47 0 8 Normal
Um, 2011 [10] Korea 46 31 46 56 89 Non-tumor Tissue Methylight
Feng, 2012 [40] China 103 82 103 40 103 Non-tumor Tissue MSP
Li, 2012 [41] China N.A. N.A. N.A. N.A. N.A. N.A. Tissue MSP
Mohamed, 2012 [42] Egypt 60 36 40 2 20 Normal Blood MSRE-qPCR
25 40 Non-tumor
Xu, 2013 [43] China 87 72 87 66 87 Non-tumor Tissue Methylight
Zhang, 2013 [44] China 123 48 48 47 83 Non-tumor Tissue MSP
6 40 Normal
Michailidi, 2014 [45] USA 27 14 27 1 17 Non-tumor Tissue MSP
Zekri, 2014 [46] Egypt 64 31 31 26 38 Non-tumor Tissue MSP
0 13 Normal
Feng, 2015 [47] China 260 214 260 101 260 Non-tumor Tissue MSP
Huang, 2015 [48] China 48 32 34 33 44 Non-tumor Tissue MSP
16 31 2 10 Normal Blood
Lin, 2015 [49] China N.A. N.A. N.A. N.A. N.A. N.A. Tissue Nested-MSP
Qu, 2015 [50] China 55 31 35 26 35 Non-tumor Tissue MSP
2 20 Normal
Kanekiyo, 2015 [51] Japan N.A. N.A. N.A. N.A. N.A. N.A. Blood qMSP
Villanueva, 2015 [52] USA 231 82 221 10 10 Normal Tissue Pyrosequencing
Dong, 2015 [53] China 584 122 190 26 234 Non-tumor Blood Methylight
2 0 0 160 Normal
Araújo, 2016 [54] Brazil 24 15 17 2 7 Non-tumor Tissue Pyrosequencing
Liu, 2017 [55] China 155 77 105 0 50 Normal Blood MSP
Mansour, 2017 [56] Egypt 121 36 41 25 40 Non-tumor Blood MSRE-qPCR
2 40 Normal
Wu, 2017 [57] USA 494 21 237 16 257 Normal Blood MSP
Pasha, 2019 [58] Egypt 300 40 10 14 100 Non-tumor Blood MSP
0 0 100 Normal
Bendary, 2019 [59] Egypt 443 108 188 22 202 Non-tumor Blood MSP
10 53 Normal
  1. Abbreviations: M, methylated; T, total; MSP, methylation-specific polymerase chain reaction; BSP, bisulfite sequencing polymerase chain reaction; qMSP, quantitative methylation-specific polymerase chain reaction; MSRE-qPCR, methylation-sensitive restriction enzyme-quantitative polymerase chain reaction; N.A., not available; Y, yes; N, no
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