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Fig. 5 | Cancer Cell International

Fig. 5

From: Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway

Fig. 5

Hsa_circ_0014235 overexpression enhanced DDP resistance and promoted cell malignant behaviors by targeting miR-550a-5p. a The expression of miR-550a-5p in cancer tissues and normal tissues was detected by qPCR. b The expression of miR-550a-5p in 16HBE, A549 and H1299 cells was detected by qPCR. c The correlation between miR-520a-5p expression and hsa_circ_0014235 expression in NSCLC tissues was analyzed by Spearman’s analysis. d, e Luciferase reporter plasmids were constructed, and dual-luciferase reporter assay was performed to verify the relationship between miR-520a-5p and hsa_circ_0014235. f The relationship between miR-520a-5p and hsa_circ_0014235 was also verified by RIP assay. g The expression of miR-520a-5p in A549 and H1299 cells transfected with oe-circ, Vector, oe-circ + miR-520a-5p mimic or oe-circ + mimic NC was detected by qPCR. h Cell proliferation, i colony formation ability, j cell cycle progression, k cell invasion, l cell migration and m cell apoptosis in these transfected cells were examined using CCK-8 assay, colony formation assay, flow cytometry assay, transwell assay, wound healing assay and flow cytometry assay, respectively. (N) The expression of MMP9, Bax, E-cadherin, Vimentin and MRP-1 in these cells was measured by western blot. *P < 0.05, **P < 0.01, ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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