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Fig. 3 | Cancer Cell International

Fig. 3

From: Extracellular vesicle-associated miR-135b and -135a regulate stemness in Group 4 medulloblastoma cells by targeting angiomotin-like 2

Fig. 3

Functional analysis of miR-135b and miR-135a in brain tumour spheroid-forming cells (BTSCs). a RT-qPCR shows significantly decreased miR-135b or miRNA-135a levels in BTSCs transfected with each anti-miRNA compared with the anti-miR-negative control (NC). b Inhibition of miR-135b or miR-135a leads to a significant reduction in cell viability in comparison with controls. c Extreme limiting dilution assay assessment shows significantly reduced tumour sphere formation ability after miR-135b or miRNA-135a inhibition. d Immunofluorescence analysis reveals that the ability of sphere formation is decreased, and the expression of nestin and musashi (MSI1) is markedly reduced by inhibition of miR-135b or miR-135a. Scale bars: 100 μm. **P < 0.01. ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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