Fig. 3

XIST acts as a sponge for miR-497-5p in CRC cells a Predicted binding sites between XIST and miR-497-5p are shown. b Luciferase activity was measured using a dual-luciferase reporter assay in cells co-transfected with pGL3-XIST-WT or pGL3-XIST-MUT vectors and miR-NC or miR-497-5p. c, d The expression of miR-497-5p was measured by qRT-PCR in CRC tissues and cell lines. e The expression of miR-497-5p was examined using qRT-PCR in HT29 and SW480 cells after transfection with si-XIST or si-NC. f XIST expression levels were determined in HT29 and SW480 cells after transfection with miR-497-5p or miRNA mimic. ^*p < 0.05; ^**p < 0.01