Fig. 5

FOXK1 is a target of miR-497-5p in CRC cells. a Predicted binding sites between FOXK1 and miR-497-5p are shown. b The luciferase activity was analyzed in HT29 and SW480 cells co-transfected with FOXK1-WT or FOXK1-MUT along with a miR-497-5p or NC mimic. c, d FOXK1 mRNA and protein expression were measured in CRC tissues and paired normal tissues by qRT-PCR or Western blot, respectively. e Expression of FOXK1 in HT29, SW480, FHC cells was measured by Western blot. f FOXK1 expression in HT29 and SW480 cells transfected with NC mimic, miR-497-5p, miR-497-5p + pcDNA, miR-497-5p + FOXK1 was detected by Western blot. ^*p < 0.05; ^**p < 0.01