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Fig. 1 | Cancer Cell International

Fig. 1

From: CircSMYD4 regulates proliferation, migration and apoptosis of hepatocellular carcinoma cells by sponging miR-584-5p

Fig. 1

CircSMYD4 with a stable ring structure was down-regulated in HCC tissues and cells. a The expression of circSMYD4 in liver cancer tissues and adjacent tissues (ANT) from 40 patients was detected by RT-qPCR. b The expression of circSMYD4 in normal cell lines and different HCC cell lines was detected by RT-qPCR. c After SNU-387 and SK-Hep1 cells were treated with RNase R, the stabilities of circSMYD4 and SMYD4 mRNA were analyzed by RT-qPCR. d Abundances of circSMYD4 mRNA in Actinomycin D-treated SNU-387 and SK-Hep1 cells at specified time points were detected by RT-qPCR. Both biological and technical replicates were examined three times. GAPDH was used as a control. The experiment was repeated for 3 times. ΔΔΔp < 0.001 vs. ANT; *p < 0.05, ***p < 0.001 vs. THLE-2; ^^^p < 0.001 vs. RNase R-. HCC hepatocellular carcinoma, RT-qPCR reverse transcription real time quantitative polymerase chain reaction

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Cancer Cell International

ISSN: 1475-2867

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