Fig. 5

Overexpression of miR-584-5p partially reversed the effects of circSMYD4 on cell proliferation and metastasis. a In SNU-387 or SK-Hep1 cells, the expression of miR-584-5p in the NC + MC, circSMYD4 + MC, NC + M and circSMYD4 + M groups was detected by RT-qPCR. U6 served as a control. b The effect of miR-584-5p on the proliferation of cells in each group was examined using clone formation assay. c The effect of miR-584-5p on the migration ability of cells in each group was examined through wound healing experiments. Magnification × 100, Scale = 50 μm. d The effect of miR-584-5p on the invasion ability of cells in each group was examined by Transwell. Magnification × 250, Scale = 50 μm. Both biological and technical replicates were examined three times. ^**p < 0.01, ^***p < 0.001 vs. NC + MC; ^{^^^}p < 0.001 vs. circSMYD4 + MC; ^##p < 0.01, ^###p < 0.001 vs. NC + M. RT-qPCR: reverse transcription real time quantitative polymerase chain reaction