Fig. 5

Exosomal miR-126 suppressed NSCLC cell malignant behaviors in vitro by targeting ITGA6. a, b The expression of ITGA6 in serum-derived exosomes transfected with miR-126, miR-NC, miR-126 + ITGA6 or miR-126 + pcDNA was determined by qRT-PCR and western blot. A549 and H460 cells were co-cultured with these transfected exosomes. In these cells, c, d cell cycle was investigated by flow cytometry assay. e The capacity of colony formation was assessed by colony formation assay. f, g Cell proliferation was assessed by MTT assay. h, i Cell migration and cell inmivasion were determined by transwell assay. j, k The expression of E-cadherin, N-cadherin and Vimentin was detected by western blot. l Cell apoptosis was monitored by flow cytometry assay. *P < 0.05