Fig. 2

Location signatures are downregulated upon Wnt activation. a Heatmap shows average (n = 3) scaled counts for both conditions of each location, additional columns show summarized mean log2FC(Cβ/wt) and mean read count across locations. Genes marked with asterix are significantly differentially expressed in the average of all locations (adjusted p-value < 0.05, Wald-Test). Internal location (int. loc.) marker genes are genes which showed the most significant (Wald-Test) upregulation in the wt samples of prSI, diSI or colon compared to all other locations’ wt samples, respectively. External location (ext. loc.) marker genes were previously reported to be specific to intestinal divisions [20, 33] or specifically enriched in the intestine based on the Human Protein Atlas [34,35,36,37]. Cell type marker genes were previously reported to be specific to differentiated intestinal cells [38,39,40]. b Our own location signature exhibited a significant downregulation upon Wnt activation in each location separately using Gene Set Enrichment Analysis GSEA preranked mode. c Cell type marker genes [38,39,40] also showed significant downregulation upon Wnt activation in Gene Set Enrichment Analysis using GSEA preranked mode