Fig. 2

Effects of NORAD overexpression on cell proliferation, invasion, apoptosis, and EMT process of PC-3 cells. PC-3 cells were transfected with 0.5 or 2 μg/mL pcDNA-NORAD expression vector, or negative control Vector (0.5 μg/mL). a Expression of NORAD was then determined by qRT-PCR after 48 h transfection. b Cell proliferation of PC-3 cells after infection for 24, 48, 72 and 96 h were detected by CCK-8 assay. c, d Transwell and Flow cytometry was performed to determine cell invasion and apoptosis in PC-3 cells after infection for 48 h, respectively. e The expression levels of N-cadherin, E-cadherin, Vimentin and Snail in PC-3 cells after infection with pcDNA-NORAD for 48 h were detected by Western blotting. The data were presented as the mean ± standard error of mean (SEM), n = 3. Student’s t-test was used for the comparison between 2 groups, and one-way analysis of variance (ANOVA) was used for the comparison among more than 2 groups in this study. *P < 0.05