Fig. 7
Downregulation of NORAD suppresses tumor growth in vivo. PC-3 cells were transfected with NORAD siRNA alone, or together with miR-30a-5p inhibitor for 48 h. Then, the transfected PC-3 cells were subcutaneously injected into the right flanks of nude mice. a The tumor volume was measured once a week for four weeks and calculated by the formula of 0.5 × Length × Width2. b Tumor-bearing mice were killed after four weeks’ cultivation, and the weight of the tumor was weighed in each group. c, d The expression levels of NORAD and miR-30a-5p were determined by qRT-PCR in tumor tissues harvested from the nude mice in each group. e, f Western blotting was used to detect the expression of RAB11A protein and the related proteins of WNT pathway (β-catenin, Cyclin D and c-Myc) in tumor tissues harvested from the nude mice in each group. The total number of mice is eighty (n = 5/group). The data were presented as the mean ± standard error of mean (SEM). Student’s t-test was used for the comparison between 2 groups in this study. *P < 0.05