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Fig. 2 | Cancer Cell International

Fig. 2

From: DLG1-AS1 is activated by MYC and drives the proliferation and migration of hepatocellular carcinoma cells through miR-497-5p/SSRP1 axis

Fig. 2

MYC activates DLG1-AS1 transcription to boost HCC cell proliferation and migration. a RT-qPCR measured mRNAs expression of transcription factor. b DLG1-AS1 expression was detected by knockdown of these expressions of transcription factor in RT-qPCR. c DNA motif of MYC and its binding sites in DLG1-AS1 promoter. d The enrichment of four parts of DLG1-AS1 promoter in anti-MYC group and anti-IgG group. e Luciferase reporter assay demonstrated the relationship between DLG1-AS1 promoter and MYC. f RT-qPCR assessed DLG1-AS1 expression in cells transfected with pcDNA3.1/DLG1-AS1. g, h Colony formation and EdU assays detected proliferation in Hep3B and HCCLM3 cells transfected with sh-NC, sh-MYC and pcDNA3.1/DLG1-AS1. i, j Apoptosis was examined in different group by flow cytometry and TUNEL. k, l Migration and invasion were evaluated in transwell among different group. **P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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