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Fig. 4 | Cancer Cell International

Fig. 4

From: DLG1-AS1 is activated by MYC and drives the proliferation and migration of hepatocellular carcinoma cells through miR-497-5p/SSRP1 axis

Fig. 4

DLG1-AS1 works as a miR-497-5p sponge to modulate SSRP1 in HCC cells. a RT-qPCR detected 8 mRNAs expression in cell transfected with miR-497-5p mimics. b Bioinformatics predicted binding sequence of miR-497-5p and SSRP1. c RNA pull down certified miR-497-5p could bind to SSRP1. d RIP assay validated SSRP1, DLG1-AS1 and miR-497-5p coexisted in RISC. e Luciferase reporter assay verified the competing relationship among DLG1-AS1 and SSRP1. f RT-qPCR and western blot were used to measure mRNA and protein levels of SSRP1 in cells transfected with NC mimics, miR-497-5p mimics and miR-497-5p mimics + pcDNA3.1/DLG1-AS1. **P < 0.01

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