Fig. 5

Activation of the mTOR/ULK1 pathway reverses the effect of Aurora-B inhibition on migration and invasion of OS cells. shAurora-B- and scrambled lentivirus-transduced 143B and HOS cells were used to detect migration and invasion. Transwell migration (a, b *P < 0.05, **P < 0.01, Scale bars: 100 μm) and invasion (c, d ***P < 0.01,*P < 0.05, **P < 0.01, Scale bars: 100 μm) assays were conducted for these cells following treatment with MHY-1485 (2 μM, 12 h) or not for 24 h. Wound healing assay (e, f **P < 0.01, *P < 0.05, ***P < 0.01, Scale bars: 100 μm) was performed in cells treated with MHY-1485 (2 μM, 12 h) for 24 h or not