Fig. 2

MiR-492 played the role of cell proliferation promotion and cell apoptosis resistance in endometrial cancer cells. a The mRNA expression of miR-492 of RL95-2, Ishikawa, MCF7, BT-474, A549 and MGC-803 cells were measured with real-time PCR. Values are means ± SD (n = 3). b Cell growth curves by MTT assay of RL95-2 cells and Ishikawa cells with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment. Results represent mean ± SD (n = 3 per group). c Cell colony formation of RL95-2 cells and Ishikawa cells with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment. Values are mean ± SD (n = 3). d EdU assay was used to detect the cell proliferation phenotype with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment for 48 h. e The protein level of Cleaved-Caspase 3, Cleaved-Caspase 9, Bax and Bcl-2 in RL95-2 cells and Ishikawa cells with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment for 48 h were detected by western blot. GAPDH was used as the internal control