Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Metapristone (RU486-derivative) inhibits endometrial cancer cell progress through regulating miR-492/Klf5/Nrf1 axis

Fig. 3

Klf5 and Nrf1 were downstream target genes of miR-492. a The mRNA level of Klf5 and Nrf1 in RL95-2 cells and Ishikawa cells with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment for 48 h were measured by real-time PCR. Values are means ± SD (n = 3). b The protein level of Klf5 and Nrf1 in RL95-2 cells and Ishikawa cells with miR-NC or miR-492 (100 nM) and si-NC or si-miR-492 (100 nM) treatment for 48 h were detected by western blot (n = 3 per group). Values are means ± SD. c Prediction of the binding sites for miR-492 in wild-type (wt) of Klf5 and Nrf1. The mutated (mut) LIFR-Klf5 and mutated (mut) LIFR-Nrf1 showed the disruption of miR-492 binding sites in RL95-2 cells and ISK cells. Values are means ± SD

Back to article page