Fig. 2
From: miR-335-5p suppresses gastric cancer progression by targeting MAPK10
miR-335-5p inhibited proliferation and promoted apoptosis in gastric cancer cells. a The effects miR-335-5p on gastric cancer cell proliferation were determined by an MTT assay after the transfection of MKN-28/SGC-7901 cells with an miR-335-5p mimic or miR-335-5p inhibitor at 24, 48, and 72 h. b The growth of MKN-28/SGC-7901 cells was detected by colony formation after transfection with the miR-335-5p mimic or inhibitor. c Cell cycle progression was evaluated in MKN-28/SGC-7901 cells transfected with miR-335-5p inhibitor-ctrl, inhibitor miR-335-5p ctrl, and mimics. After 48 h, the cell cycle distribution was analyzed by flow cytometry. The histogram indicates the percentages of cells in G0/G1, S, and G2/M phases. d Apoptosis was detected by annexin-V/propidium iodide combined labeling flow cytometry in MKN-28/SGC-7901 cells 48 h after transfection with miR-335-5p inhibitor-ctrl, inhibitor, miR-335-5p ctrl, and mimics. Apoptosis was evaluated as the percentage of apoptotic cells (*P < 0.05, **P < 0.01, and ***P < 0.005)