Fig. 4

MAPK10 is a direct target of miR-335-5p in gastric cancer cell lines. a Correlation between MAPK10 expression and miR-335-5p expression in gastric cancer based on TCGA data. b A luciferase assay was performed using HEK293 cells in which miR-335 was co-transfected with the pGLO-MAPK10 wild-type or pGLO-MAPK10 mutant vector. c miR-335-5p is highly conserved across species and has binding sites within the 3′-UTR of human MAPK10. d mRNA expression levels of MAPK10 were measured by qRT-PCR after transfection with miR-335-5p mimics, miR-335-5p inhibitor, or their negative controls in MKN-28 and SGC-7901 cells. e Protein expression levels of MAPK10 were measured by western blotting after transfection with miR-335-5p mimics, miR-335-5p inhibitor, or their respectively negative controls in MKN-28 and SGC-7901 cells (*P < 0.05, **P < 0.01, and ***P < 0.005)