Fig. 6
From: miR-335-5p suppresses gastric cancer progression by targeting MAPK10
Inhibition of MAPK10 suppressed GC progression. a mRNA and protein expression levels of MAPK10 in various GC and GES-1 cells. b MAPK10 protein expression in GC tissues vs counterparts' tissues was confirmed by using western blotting. c Expression levels of MAPK10 were measured by qRT-PCR in MKN-28 and SGC-7901 cells transfected with siMAPK10. d An MTT assay was performed to determine the growth of gastric cancer cells treated with siMAPK10 or a negative control (si-ctrl). e A colony formation assay was performed several days after the transfection of gastric cancer cells with siMAPK10 or a negative control (si-ctrl). f The cell cycle distribution was determined in gastric cancer cells 48 h after transfection with siMAPK10 by propidium iodide staining and flow cytometry. The histogram indicates the percentages of cells in G0/G1, S, and G2/M cell cycle phases. g Apoptosis was determined in gastric cancer cells at 48 h after transfection with siMAPK10 (*P < 0.05, **P < 0.01, and ***P < 0.005)