Fig. 4

CBR3-AS1 promotes LAD cell proliferation by target Wnt/β-catenin signaling. a A549, H23 or H1975 cells were stably transfected with control shRNA or CBR3-AS1 siRNA or transfected with pcDNA3.1-CBR3-AS1 and examined the expression of CBR3-AS1, c-Myc, LGR5 and MMP-7 by qRT-PCR assays. Each bar represents the mean ± SD for biological triplicate experiments. **P < 0.01, one-way ANOVA. b Colony formation assays with A549 cells stably expressing control shRNA or CBR3-AS1 shRNA and cultured in the absence or presence of 25 mM LiCl for 2 weeks. c Growth viability assay with A549 cells stably expressing control shRNA or CBR3-AS1 shRNA and cultured in the absence or presence of 25 mM LiCl for 8 days. Each bar represents the mean ± SD for biological triplicate experiments. 0.01 < *P < 0.05, **P < 0.01, one-way ANOVA. d Cellular viability assay by CCK8 kit with A549 cells stably expressing control shRNA or CBR3-AS1 shRNA and cultured in the absence or presence of 25 mM LiCl. Each bar represents the mean ± SD for biological triplicate experiments. **P < 0.01, one-way ANOVA. e Cellular viability assay by CCK8 kit with A549 cells stably expressing control shRNA or β-catenin shRNA or co-transfected with β-catenin and CBR3-AS1 shRNAs. Each bar represents the mean ± SD for biological triplicate experiments. ^#P > 0.05, **P < 0.01, one-way ANOVA. f Cellular viability assay by CCK8 kit with A549 cells stably expressing control shRNA or CBR3-AS1shRNA or co-transfected with FLAG-β-catenin and CBR3-AS1 shRNAs. Each bar represents the mean ± SD for biological triplicate experiments. ^#P > 0.05, one-way ANOVA.