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Fig. 3 | Cancer Cell International

Fig. 3

From: Inhibition of cancer cell-derived exosomal microRNA-183 suppresses cell growth and metastasis in prostate cancer by upregulating TPM1

Fig. 3

miR-183 targets and negatively regulates the expression of TPM1 in prostate cancer. a Prediction of miR-183 downstream target genes, blue, red, and green indicates prediction results of miRDB, mirDIP and miRWalk database respectively, yellow indicates the top 100 significantly downregulated genes in GSE30994 profile, the region of number 5 referring the intersection of 4 data sets. b Expression of TPM1 in prostate cancer and normal tissues in GEPIA website. c The binding sites of miRNA and TPM1-3′UTR predicted by the software. d The interaction between miR-183 and TPM1 assessed by dual-luciferase reporter gene assay. e The expression of TPM1 in RWPE-1, LNCaP and PC3 cell lines measured by Western blot analysis. *p < 0.05 vs.. RWPE-1 cells, #p < 0.05 vs. LNCAP cells. f The expression of TPM1 measured by Western blot analysis. *p < 0.05 vs. mimic-NC, #p < 0.05 vs. inhibitor-NC group. The miR-183-mimic group, miR-183-inhibitor group, mimic-NC group, and inhibitor-NC group refer to LNCaP cells respectively transfected with miR-183 mimic, miR-183 inhibitor, mimic-NC, inhibitor-NC, respectively. Cell experiment was repeated three times

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