Fig. 5

PC3 cells-derived exosomal miR-183 downregulates TPM1. a The secretion of exosomes observed under an electron microscope (× 5000). b Quantification of HSP70 and CD63 in exosomes detected by Western blot. c The expression of f miR-183 and TPM1 in PC3 cells detected by RT-qPCR assay. d The expression of f miR-183 analyzed by RT-qPCR assay. e The expression of TPM1 in LNCaP cells evaluated by RT-qPCR assay. f The expression of TPM1 in LNCaP cells evaluated by Western blot assay. *p < 0.05 vs. exo-mimic-NC group, ^#p < 0.05 vs. exo-inhibitor-NC group. The exo-mimic-NC group, exo-miR-183-mimic group, exo-inhibitor-NC group, and miR-183-inhibitor group refer to exosome derived from PC3 cells respectively transfected with mimic-NC, exo-miR-183-mimic, inhibitor-NC and miR-183-inhibitor. The exosomes derived from the transfected PC3 cells were co-cultured with LNCAP cells Measurement data were expressed as mean ± standard deviation. Comparison between two groups was analyzed using independent sample t-test. Cell experiment was repeated three times