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Table 1 Primer sequences for RT-qPCR

From: Inhibition of cancer cell-derived exosomal microRNA-183 suppresses cell growth and metastasis in prostate cancer by upregulating TPM1

Gene

Primer sequence (5′-3′)

miR-183

Forward: 5′-CGCGGTATGGCACTGGTAGA-3′

Reverse: 5′-AGTGCAGGGTCCGAGGTATTC-3′

TPM1

Forward: 5′-GTGGGGAAAACACATACAAAAAG-3′

Reverse: 5′-CTTCCTGTTGACTCTATCATTGG-3′

GAPDH

Forward: 5′-CCTGGCCAAGGTCATCCATG-3′

Reverse: 5′-GGAAGGCCATGCCAGTGAGC-3′

U6

Forward: 5′-CTCGCTTCGGCAGCACA-3′

Reverse: 5′-AACGCTTCACGAATTTGCGT-3′

  1. RT-qPCR reverse transcription quantitative polymerase chain reaction, miR-183 microRNA-183, TPM1 tropomyosin-1, GAPDH glyceraldehyde-3-phosphate dehydrogenase, F forward, R reverse