Fig. 3
From: YAP manipulates proliferation via PTEN/AKT/mTOR-mediated autophagy in lung adenocarcinomas
Autophagy in A549 and H1299 cell manipulated by YAP gene. a Analysis of autophagy-related protein expression in A549 cell manipulated by YAP1 gene. Cells were analyzed by immunoblotting with indicated antibodies. b Autophagy substrate proteins p62 and autophagy marker proteins LC3I/II were detected in A549/H1299-siYAP cellular extracts by Western blot. c Immunofluorescence analysis of autolysosomes puncta in A549 and H1299 cells transfected with siYAP. Confocal microscopy images showing cellular localization of autophagic dots in A549/H1299-siYAP cells. The GFP stain (green puncta) was on behalf of the initial process of autophagy and the mRFP (red puncta) indicated the late process of autophagy. Magnification is × 400. d Quantification of autophagy activity was analyzed from 10 random visual fields for each group. At least three different visual fields containing at least 20 cells were counted for each condition and shown in the graph. Data were shown as the Mean ± SD. Statistical analysis was calculated by one-way ANOVA. Scale bars = 25 µm (*P < 0.05, **P < 0.01)