Fig. 4

YAP induced activation of Akt/mTOR signaling pathway via suppressing PTEN in a Hippo-pathway-dependent manner. a. Following transfected with siYAP or pcDNA3.1-YAP in A549, Western blot measured the level of proteins associated with Akt/mTOR signaling pathway. b–d Representative immunofluorescence of A549 cells transfected with siNC, siYAP, pcDNA3.1 or pcDNA3.1-YAP was observed by Inversed Fluorescent Microscope. Fluorescent staining of YAP, pAKT and pS6K was shown as green stain and fluorescence intensity indicated the relative amount in the cells. Magnification is × 200. e Western blot showed the expressive levels of AKT, pAKT, S6K, pS6K, and PTEN after transfection with or without shPTEN in A549-siYAP, with or without pcDNA3.1-PTEN in A549-pcDNA3.1-YAP, respectively. f Western blot showed the expressive levels of AKT, pAKT, S6K, pS6K, and YAP after knockdown LATS, which being a upstream of YAP and a core factor in Hippo pathway