Fig. 5
From: YAP manipulates proliferation via PTEN/AKT/mTOR-mediated autophagy in lung adenocarcinomas
The transformation of autophagy in A549/H1299-siYAP after treated with 3-MA. a, b Theproliferationability of A549/H1299-siYAP and A549/H1299-siYAP+3-MA was determined by CCK-8 assay. c A549/H1299-siYAP cells treated with 3-MA were lysated. Autophagy substrate proteins p62 and the conversion of LC3B-I to LC3B-II were detected by the Western blot. d, e Confocal microscopy images showing cellular localization of autophagic dots in A549/H1299-siYAP or A549/H1299-siYAP + 3MA cells. The GFP stain (green puncta) was on behalf of the initial process of autophagy and the mRFP (red puncta) indicated the late process of autophagy. Magnification is × 400. Quantification of autophagy activity was analyzed from 10 random visual fields for each group and shown in the graph. Data were shown as the Mean ± SD (one-way analysis, *P < 0.05, **P < 0.01, ***P < 0.001)