Fig. 2

Si-HOXA-AS2 inhibited cell viability, cell proliferation and cell adhesion, but it induced cell apoptosis in glioblastoma cells. a HOXA-AS2 expression in glioblastoma tissues and non-tumor tissues was analyzed using qRT-PCR. N = 33. b HOXA-AS2 expression in glioblastoma cell lines (U251, U87, A172, SHG44 and SNB19) and normal human astrocytes cell line (NHA) was analyzed using qRT-PCR. c The intracellular distribution of HOXA-AS2 was identified using a subcellular fractionation location assay. d The transfection efficiency of si-HOXA-AS2 was verified in U87 and U251 cell lines by qRT-PCR. e The viability of the transfected U87 and U251 cells was measured after performing the CCK-8 assay. f The proliferation of the transfected U87 and U251 cells was measured using the BrdU assay. g The adhesion ability of the transfected U87 and U251 cells was evaluated using the cell adhesion assay. h The apoptosis rate of the transfected U87 and U251 cells was evaluated using flow cytometry. i The Twist, Slug, Vimentin, MMP-2 protein expression level of the transfected U87 and U251 cells was evaluated using western blot assay. NC, negative control. Si-LNC, si-HOXA-AS2. The cells in the blank group without any treatments. The data were presented in the form of mean ± SD, and three independent experiments were performed. *P < 0.05, **P < 0.001