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Fig. 8 | Cancer Cell International

Fig. 8

From: LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis

Fig. 8

MiR-885-5p directly targeted RBBP4 by binding to its 3′UTR. a The potential binding site between miR-885-5p and RBBP4 was predicted using TargetScan Human 7.2. b The potential binding site between miR-885-5p and the 3′UTR of RBBP4 was demonstrated using the dual-luciferase assay. NC, negative control. Mimic, miR-885-5p mimic. c The interaction between RBBP4 and miR-885-5p was evaluated using the RNA pull-down assay. Bio-NC, biotinylated negative control. Bio-miR-885-5p, biotinylated miR-885-5p. d RBBP4 expression in glioblastoma tissues and normal tissues was detected by qRT-PCR. N = 33. e The correlation analysis of miR-885-5p and RBBP4. f RBBP4 expression in glioblastoma cell lines (U251 and U87) and normal human astrocytes cell line (NHA) were identified using qRT-PCR. g The expression of RBBP4 protein in glioblastoma cell lines (U251 and U87) and normal human astrocytes cell line (NHA) were detected with the western blot kit. h The expression of RBBP4 mRNA increased by miR-885-5p inhibitor in U87 and U251 cells. i The expression of RBBP4 protein increased by miR-885-5p inhibitor in U87 and U251 cells. NC, negative control. Inhibitor, miR-885-5p inhibitor. The cells in the blank group without any treatments. The data were presented in the form of mean ± SD, and three independent experiments were performed. *P < 0.05, **P < 0.001

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