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Fig. 3 | Cancer Cell International

Fig. 3

From: HCG11 up-regulation induced by ELK4 suppressed proliferation in vestibular schwannoma by targeting miR-620/ELK4

Fig. 3

MiR-620 down-regulation suppressed the growth of VS. a ELK4 expression was examined by RT-qPCR in cells transfected with pcDNA3.1/HCG11. b FISH experiments were built to judge the place of HCG11 in VS cells. c Venn diagram showed miRNAs could both bind to ELK4 and HCG11. d 14 miRNAs expressions were examined in cells transfected with pcDNA3.1/HCG11. e Bioinformatics projected the binding sequences between HCG11 and ELK4. f MiR-620 expression was appraised in VS cells. g RNA pull down was constructed to confirm the relationship between HCG11 and miR-620. h RT-qPCR was used to test the expression of miR-620 and luciferase reporter assays were performed to verify the combination between miR-620 and HCG11. i RT-qPCR was used to assess the expression of miR-620 in cells treated with miR-620 inhibitor. j, k CCK8 and EdU assays were built to probe the cell proliferative capacities in cells transfected with pcDNA3.1/HCG11 and miR-620 inhibitor. l, m The apoptosis rate was estimated in flow cytometry analysis and TUNEL in cells received transfection with pcDNA3.1/HCG11 and miR-620 inhibitor. *P < 0.05, **P < 0.01

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