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Fig. 4 | Cancer Cell International

Fig. 4

From: HCG11 up-regulation induced by ELK4 suppressed proliferation in vestibular schwannoma by targeting miR-620/ELK4

Fig. 4

HCG11 repressed the process of VS by up-regulating ELK4 expression. a The binding sequences between miR-620 and ELK4 were displayed by bioinformatics. b RIP assays were built to corroborate ELK4, HCG11 and miR-620 coexisted in RISCs. c Luciferase reporter assays were performed to verify the competing relationship between ELK4 and HCG11. d RT-qPCR was carried out to probe the expression of ELK4 in VS cell lines. e Sh-ELK4#1 was transfected into cells and the expression of ELK4 was tested by RT-qPCR. f, g CCK8 and EdU assays were performed to measure cell proliferation in cells treated with pcDNA3.1/HCG11 and sh-ELK4#1. h, i Apoptosis was determined by flow cytometry analysis and TUNEL in cells transfected with pcDNA3.1/HCG11 and sh-ELK4#1. *P < 0.05, **P < 0.01

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