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Fig. 3 | Cancer Cell International

Fig. 3

From: Berberine reduces temozolomide resistance by inducing autophagy via the ERK1/2 signaling pathway in glioblastoma

Fig. 3

Berberine promotes TMZ-induced autophagy in TMZ-R cells. a and b The indicated protein level was analyzed by western blotting in parental and TMZ resistance cells. LC3 II was normalized to LC3 I. c TMZ-R U87 cells were transfected with GFP-LC3 plasmid, followed by treatment with 100 μM TMZ with or without 10 μM berberine for 24 h. The numbers of GFP-LC3 puncta were quantified with confocal microscopy. d TMZ-R U251 cells were transfected with GFP-LC3 plasmid, followed by treatment with 100 μM TMZ with or without 10 μM berberine for 24 h. The numbers of GFP-LC3 puncta were quantified with confocal microscopy. e and f TMZ resistance cells were treated with 100 μM TMZ with or without 10 μM berberine as indicated for 24 h. Indicated proteins level were analyzed by western blotting. LC3 II was normalized to LC3 I. g and h TMZ resistance cells were treated with 100 μM TMZ and 10 μM berberine as indicated time points. Indicated proteins level were analyzed by western blotting. LC3 II was normalized to LC3 I. BBR: berberine. Results were presented as means ± SD from three independent experiments. *, P < 0.05

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