Fig. 6
Berberine resensitizes TMZ to TMZ-R tumors in vivo. a Nude mice were injected s.c. with 4 × 106 TMZ-R cells. After 1 week, mice were treated with TMZ, berberine or their combination. Tumor volume was calculated and plotted with p values, n = 6 in each group. b Tumor weight at the end of the experiment. c The indicated proteins in randomly selected tumors were analyzed by Western blotting. LC3 II was normalized to LC3 I. d Paraffin-embedded sections of TMZ-R tumor tissues from mice treated were analyzed by active caspase 3 staining. e Paraffin-embedded sections of TMZ-R tumor tissues from mice treated were analyzed by LC3B staining. BBR: berberine. Results were presented as means ± SD from three independent experiments. *, P < 0.05